TY - JOUR
KW - article
KW - Ethyl carbamate
KW - Mechanism research
KW - Musalais
KW - Toxicity prediction
KW - Transcriptomics sequencing
KW - Wnt protein
KW - Wnt signaling
KW - alcohol dehydrogenase
KW - animal experiment
KW - animal model
KW - animal tissue
KW - carcinogenesis
KW - concentration (parameter)
KW - controlled study
KW - cyclin D1
KW - cytochrome P450
KW - fermented beverage
KW - gene identification
KW - glutathione transferase A1
KW - glutathione transferase A2
KW - glutathione transferase A5
KW - glutathione transferase P1
KW - liver toxicity
KW - nephrotoxicity
KW - nonhuman
KW - p53 signaling
KW - protein analysis
KW - protein metabolism
KW - protein p53
KW - rat
KW - regulatory mechanism
KW - sequence analysis
KW - toxicity testing
KW - transcriptomics
KW - upregulation
KW - urethan
AU - Weihua Wang
AU - ZhanJiang Han
AU - Dongqi Guo
AU - Yanju Xiang
AB - Introduction: Musalais is a traditional fermented wine produced in southern Xinjiang (a province of China) and is protected as a form of national intangible cultural heritage. However, ethyl carbamate (EC), which is naturally produced during the fermentation pro-cess, has been shown to induce carcinogenesis and was classified as a group 2A carcinogen by The World Health Organization’s International Agency for Research on Cancer. Methods: In this work, rats were treated with musalais containing EC at varying contents (0.1, 1, or 10 mg/kg). To evaluate the toxicity of EC in musalais, the liver and kidney of the rats were subjected to transcriptomics sequencing. Differentially expressed genes (DEGs) between treated and untreated rats were identified, and Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis were performed on these genes to investigate the biological functions affected by EC in musalais. Results: The results demonstrated that high EC content in musalais is possibly involved in the regulation of cytochrome P450 metabolism, chemical carcinogenesis, metabolism of xenobiotics by cytochrome P450, Wnt signaling, and p53 signaling by targeting Mgst1, Gstp1, Gsta5, Gsta1, Adh1, Gsta2, and Ccnd1, thereby inducing cancer. Conclusion: The present work predicted the potential carcinogenic mechanism of high EC content in musalais, providing a reference for its safety evaluation.
DO - 10.2147/OTT.S282125
N1 - Publisher: Dove Medical Press Ltd
N2 - Introduction: Musalais is a traditional fermented wine produced in southern Xinjiang (a province of China) and is protected as a form of national intangible cultural heritage. However, ethyl carbamate (EC), which is naturally produced during the fermentation pro-cess, has been shown to induce carcinogenesis and was classified as a group 2A carcinogen by The World Health Organization’s International Agency for Research on Cancer. Methods: In this work, rats were treated with musalais containing EC at varying contents (0.1, 1, or 10 mg/kg). To evaluate the toxicity of EC in musalais, the liver and kidney of the rats were subjected to transcriptomics sequencing. Differentially expressed genes (DEGs) between treated and untreated rats were identified, and Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis were performed on these genes to investigate the biological functions affected by EC in musalais. Results: The results demonstrated that high EC content in musalais is possibly involved in the regulation of cytochrome P450 metabolism, chemical carcinogenesis, metabolism of xenobiotics by cytochrome P450, Wnt signaling, and p53 signaling by targeting Mgst1, Gstp1, Gsta5, Gsta1, Adh1, Gsta2, and Ccnd1, thereby inducing cancer. Conclusion: The present work predicted the potential carcinogenic mechanism of high EC content in musalais, providing a reference for its safety evaluation.
SP - 1401
EP - 1416
TI - Renal Transcriptomics Reveals the Carcinogenic Mechanism of Ethyl Carbamate in Musalais
UR - https://www.scopus.com/inward/record.uri?eid=2-s2.0-85102299444&doi=10.2147%2fOTT.S282125&partnerID=40&md5=f4eb9112e9b8faeaad9df18086038a3d
VL - 14
SN - 11786930 (ISSN)
ER -