TY - JOUR KW - Fresh cheese KW - High-intensity ultrasound KW - Microbiological control KW - Nutritional quality AU - Luis Carrillo-Lopez AU - Monica Juarez-Morales AU - Ivan Garcia-Galicia AU - Alma Alarcon-Rojo AU - Mariana Huerta-Jimenez AB - High-intensity ultrasound could be an alternative to pasteurization for cheeses made with fresh raw milk, the properties of which must be preserved as part of their intangible cultural heritage, such as Panela cheese in Mexico. This research aimed to study the effect of the amplitude (50\% and 100\%) and application time (0, 5, and 10 min) of ultrasound treatment of fresh raw milk, on the yield and microbiological and physicochemical qualities of Panela cheese after 24 h of storage at 4 ◦C. The yield was increased to 24.29\% with 10 min of ultrasonication, although the amount of exudate was higher in the ultrasonic product than in the control (20.33\%). As the ultrasonication time increased, the yellowness (b*) increased significantly, while the hue angle decreased (with values close to 90◦), resulting in evident yellow tones in cheeses made with milk treated for 10 min. The pH significantly increased from 6.6 to 6.74 with 5 min of ultrasound, but decreased to 6.37 with 10 min of ultrasonication. Although no significant differences were found in fat content, the protein significantly increased with 5 min of sonication, but it decreased markedly when ultrasound was applied for 10 min. Ultrasound treatment with amplitudes of 50\% effectively decreased the counts of coliform bacteria regardless of ultrasonication time. However, the mesophilic bacteria increased by a 0.9 log with an amplitude of 100\% and 10 min treatment. The results showed that ultrasound improved the yield and microbial, nutritional, and physicochemical properties of Panela cheese. © 2020 by the authors. DO - 10.3390/foods9030313 M1 - 3 N1 - Publisher: MDPI Multidisciplinary Digital Publishing Institute N2 - High-intensity ultrasound could be an alternative to pasteurization for cheeses made with fresh raw milk, the properties of which must be preserved as part of their intangible cultural heritage, such as Panela cheese in Mexico. This research aimed to study the effect of the amplitude (50\% and 100\%) and application time (0, 5, and 10 min) of ultrasound treatment of fresh raw milk, on the yield and microbiological and physicochemical qualities of Panela cheese after 24 h of storage at 4 ◦C. The yield was increased to 24.29\% with 10 min of ultrasonication, although the amount of exudate was higher in the ultrasonic product than in the control (20.33\%). As the ultrasonication time increased, the yellowness (b*) increased significantly, while the hue angle decreased (with values close to 90◦), resulting in evident yellow tones in cheeses made with milk treated for 10 min. The pH significantly increased from 6.6 to 6.74 with 5 min of ultrasound, but decreased to 6.37 with 10 min of ultrasonication. Although no significant differences were found in fat content, the protein significantly increased with 5 min of sonication, but it decreased markedly when ultrasound was applied for 10 min. Ultrasound treatment with amplitudes of 50\% effectively decreased the counts of coliform bacteria regardless of ultrasonication time. However, the mesophilic bacteria increased by a 0.9 log with an amplitude of 100\% and 10 min treatment. The results showed that ultrasound improved the yield and microbial, nutritional, and physicochemical properties of Panela cheese. © 2020 by the authors. TI - The Effect of High-Intensity Ultrasound on the Physicochemical and Microbiological Properties of Mexican Panela Cheese UR - https://www.scopus.com/inward/record.uri?eid=2-s2.0-85081595595&doi=10.3390%2ffoods9030313&partnerID=40&md5=c7bef725764dafd2b9cdc8cd264213c1 VL - 9 SN - 23048158 (ISSN) ER -