03115nas a2200493   4500000000100000000000100001008004100002653001300043653001300056653002100069653002200090653001100112653002100123653001500144653003800159653001900197653002600216653003300242653003400275653002700309653001900336653002700355653001900382653002200401653002900423653001300452653001200465653001400477653003400491653001200525100001100537700001200548700001000560700001300570700001200583700001300595700001100608245011800619856014600737300001400883490000700897520169700904022002002601                                       d10aBacteria10aCoatings10aCondensed tannin10aCondensed tannins10aDyeing10aDyeing technique10aFlavonoids10aHigh resolution mass spectrometry10aHydrogen bonds10aInfrared spectroscopy10aIntangible cultural heritage10aIntangible cultural heritages10aInteraction mechanisms10aIron compounds10aIron reducing bacteria10aIron reduction10aMass spectrometry10aMicrobial iron reduction10aProteins10aQuinone10aReduction10aShewanella decolorationis S1210aTannins1 aY. Pan1 aX. Yang1 aM. Xu1 aH. Liang1 aH. Xiao1 aD. Huang1 aG. Sun00aMechanism of dyeing technique of Xiang-yun-sha and bioaugmentation of its coating mud with iron reducing bacteria  uhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85102906903&doi=10.1360%2fN092018-00136&partnerID=40&md5=b5c74b369aac08bbb9ded6273a781ba7  a1012-10200 v483 aThe dyeing technique of Xiang-yun-sha, with a history of more than 600 years, is a kind of unique hand-made dyeing process that combines both plant dyeing (immersed with Juliang extract) and mineral dyeing (mud coating). It has been included in the national intangible cultural heritage. However, the scientific mechanism of this heritage has not been systematically explained. In this study, the composition and structure of Juliang were analyzed with ionizing-spray high-resolution mass spectrometry and infrared spectroscopy, and the interaction mechanism of the Juliang with silk protein was explored. The mud-coating process was explained from the molecular level through a chemical simulation method, and the maintenance effect of microbes on characteristics of river mud was examined. Results showed that there are phenol and quinone of catechin condensed tannin in the Juliang extract, and they were combined with silk protein peptide bonds through multi-sites hydrogen bonds. The gial pigments on the silk surface after exposure further reacted with Fe(II) in the mud to form soluble condensed tannin complex, and then this complex was rapidly oxidized to form black precipitate containing Fe(III). Eventually, lustrous dark brown was formed on sun side, brown on the other side. Further, it was found that microbial iron reduction played a key role on maintaining the mud properties, because the addition of Shewanella decolorationis S12 with iron reducing function can significantly improve the dyeing performance of unqualified mud. This study provides reliable scientific support to protect inheritance of dyeing technique and promote the standardized production of the industry.  a16747259 (ISSN)