03199nas a2200613   4500000000100000008004100001653001200042653002000054653002300074653001300097653002400110653003100134653001600165653001800181653002600199653002200225653001700247653001800264653001900282653003000301653002100331653001400352653002000366653002300386653002400409653003100433653003100464653003100495653003100526653001900557653001900576653001300595653001800608653002100626653002300647653001600670653000800686653002500694653002200719653002100741653002000762653001700782653001200799100001600811700001800827700001500845700001600860245009200876856014400968300001401112490000701126520143201133022002002565                                       d10aarticle10aEthyl carbamate10aMechanism research10aMusalais10aToxicity prediction10aTranscriptomics sequencing10aWnt protein10aWnt signaling10aalcohol dehydrogenase10aanimal experiment10aanimal model10aanimal tissue10acarcinogenesis10aconcentration (parameter)10acontrolled study10acyclin D110acytochrome P45010afermented beverage10agene identification10aglutathione transferase A110aglutathione transferase A210aglutathione transferase A510aglutathione transferase P110aliver toxicity10anephrotoxicity10anonhuman10ap53 signaling10aprotein analysis10aprotein metabolism10aprotein p5310arat10aregulatory mechanism10asequence analysis10atoxicity testing10atranscriptomics10aupregulation10aurethan1 aWeihua Wang1 aZhanJiang Han1 aDongqi Guo1 aYanju Xiang00aRenal Transcriptomics Reveals the Carcinogenic Mechanism of Ethyl Carbamate in Musalais  uhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85102299444&doi=10.2147%2fOTT.S282125&partnerID=40&md5=f4eb9112e9b8faeaad9df18086038a3d  a1401-14160 v143 aIntroduction: Musalais is a traditional fermented wine produced in southern Xinjiang (a province of China) and is protected as a form of national intangible cultural heritage. However, ethyl carbamate (EC), which is naturally produced during the fermentation pro-cess, has been shown to induce carcinogenesis and was classified as a group 2A carcinogen by The World Health Organization’s International Agency for Research on Cancer. Methods: In this work, rats were treated with musalais containing EC at varying contents (0.1, 1, or 10 mg/kg). To evaluate the toxicity of EC in musalais, the liver and kidney of the rats were subjected to transcriptomics sequencing. Differentially expressed genes (DEGs) between treated and untreated rats were identified, and Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis were performed on these genes to investigate the biological functions affected by EC in musalais. Results: The results demonstrated that high EC content in musalais is possibly involved in the regulation of cytochrome P450 metabolism, chemical carcinogenesis, metabolism of xenobiotics by cytochrome P450, Wnt signaling, and p53 signaling by targeting Mgst1, Gstp1, Gsta5, Gsta1, Adh1, Gsta2, and Ccnd1, thereby inducing cancer. Conclusion: The present work predicted the potential carcinogenic mechanism of high EC content in musalais, providing a reference for its safety evaluation.  a11786930 (ISSN)